There were no surgeries in this week, but I saw several patients with Dr. Lipkin. Some of them have colorectal cancers (CRC) with inflammation bowel diseases (IBD). IBD, often characterized by conditions such as ulcerative colitis and Chron's disease, increases the risk of developing colorectal cancer. (However, ulcerative colitis increases risk more than Chron's disease.)In general, the longer a person has had IBD, the greater his or her chance of developing colorectal cancer. This is because inflamed areas of the colon can give rise to abnormally-developed cells, which in time, can give rise to cancer cells. We had one patient of CRC & IBD registered for CRC surgery and I am curious to get the tumor tissues for histological analysis and primary culture.
Research project goes slowly but progressively. I use the primary CRC lines for small molecule screening and identified some KRAS targeting components. While the KRAS mutation sequencing results are not good due to the bad DNA sample qualities. I will repeat DNA exaction to improve the quality by more PCR production and gel elution. In the other hand, we consult gene sequencing / expression expert, Dr. Zeynep Gumus about the whole genome / exon sequencing for these highly metastatic primary CRC lines.
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